1 Scope
This document specifies requirements and recommendations to laboratories that perform
extraction of polymerase chain reaction (PCR) quality deoxyribonucleic acid (DNA)
from cottonseed, cotton leaf and raw material derived therefrom, that is sufficient
for the purpose of PCR analysis.
This document is applicable to:
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a) identifying cotton raw material from which PCR quality DNA can be extracted;
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b) specifying a method for effective DNA extraction from cotton and cotton-derived raw materials;
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c) specifying the cotton-specific marker(s) to be used as controls for PCR amplification of DNA.
A PCR result obtained from analysis of cottonseed, cotton leaf and to some extant
raw materials derived therefrom can only indicate that it is not derived from pure
genetically modified organism (GMO)-derived cotton. Admixtures of GMO-derived cotton
cannot be detected for cotton fibre and cotton fibre-derived materials.
This document does not apply to bulk sampling of the seed, bale or processed fabric
and yarn. A recommended sampling method is given in ISO 6497[5]. General guidance for the sampling of bulk materials or for cotton-based products
is available in standards such as ASTM D1441-12[6] and CEN/TS 15568[7].